Type II restriction endonucleases fromBacillus sphaericus
نویسندگان
چکیده
منابع مشابه
Structure and function of type II restriction endonucleases.
More than 3000 type II restriction endonucleases have been discovered. They recognize short, usually palindromic, sequences of 4-8 bp and, in the presence of Mg(2+), cleave the DNA within or in close proximity to the recognition sequence. The orthodox type II enzymes are homodimers which recognize palindromic sites. Depending on particular features subtypes are classified. All structures of res...
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This article continues the series of Surveys and Summaries on restriction endonucleases (REases) begun this year in Nucleic Acids Research. Here we discuss 'Type II' REases, the kind used for DNA analysis and cloning. We focus on their biochemistry: what they are, what they do, and how they do it. Type II REases are produced by prokaryotes to combat bacteriophages. With extreme accuracy, each r...
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The endonucleases from the Type IIB restriction-modification systems differ from all other restriction enzymes. The Type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex. The fact that ...
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Restriction and modification are two opposing activities that are used to protect bacteria from cellular invasion by DNA (e.g. bacteriophage infection). Restriction activity involves cleavage of the DNA; while modification activity is the mechanism used to "mark" host DNA and involves DNA methylation. The study of Type I restriction enzymes has often been seen as an esoteric exercise and this ...
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P. Kurian, G. Dunston, J. Lindesay 1. Department of Physics and Astronomy, Howard University, Washington, District of Columbia 20059 2. Computational Physics Laboratory, Howard University, Washington, District of Columbia 20059 3. National Human Genome Center, Howard University College of Medicine, Washington, District of Columbia 20059 4. Department of Microbiology, Howard University College o...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1986
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1986.tb01800.x